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Rab Gtpases: Methods and Protocols

Rab Gtpases: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1298

BiologyChemistry

ISBN10: 1493925687
ISBN13: 9781493925681
Publisher: Springer Nature
Published: Mar 25 2015
Pages: 358
Weight: 1.91
Height: 0.88 Width: 7.00 Depth: 10.00
Language: English

This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

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