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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Quantification of Amlodipine and Indapamide in Human Plasma by HPLC

Quantification of Amlodipine and Indapamide in Human Plasma by HPLC

Pethani, Trupesh
Thumar, Mayuri
Varsani, Ravi

Paperback

Medical Reference

ISBN10: 6202438363
ISBN13: 9786202438360
Publisher: LAP Lambert Academic Publishing
Published: Sep 18 2025
Pages: 104
Weight: 0.33
Height: 0.25 Width: 6.00 Depth: 9.00
Language: English
A simple, sensitive, and selective HPLC method was developed and validated for the simultaneous estimation of Amlodipine besylate (AML) and Indapamide (IND) in human plasma for application in pharmacokinetic, bioequivalence, and bioavailability studies. AML, a calcium channel blocker, and IND, a thiazide-like diuretic, are widely prescribed in combination for hypertension management. The method employed liquid-liquid extraction using diethyl ether and dichloromethane, followed by chromatographic separation on a C18 column with a methanol-octane sulfonic acid mobile phase (79:21 v/v) at a flow rate of 1.2 mL/min and UV detection at 240 nm. The retention times for AML, IND, and the internal standard (carbamazepine) were 8.9, 2.9, and 3.6 min respectively. The method was linear over 0.075-15 ng/mL for AML and 0.3-60 ng/mL for IND, with correlation coefficients >=0.998. Validation as per USFDA guidelines demonstrated accuracy, precision, recovery, stability, and selectivity within acceptance criteria. This validated HPLC method is robust and suitable for routine analysis of AML and IND in plasma, supporting clinical and regulatory studies.

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