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Piwi-Interacting Rnas: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1093
ISBN13: 9781627036931
Publisher: Springer Nature
Published: Nov 1 2013
Pages: 249
Weight: 1.49
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
1. Chromatin Immunoprecipitation Assay of Piwi in Drosophila
Hang Yin and Haifan Lin
2. Drosophila Germline Stem Cell for in vitro Analyses of PIWI-Mediated RNAi
Yuzo Niki, Takuya Sato, Takashi Yamaguchi, Ayako Saisho, Hiroshi Uetake and Hidenori Watanabe
3. RNAi and Overexpression of Genes in Ovarian Somatic Cells
Kuniaki Saito
4. Making piRNAs in vitro
Shinpei Kawaoka, Susumu Katsuma and Yukihide Tomari
5. A Framework for piRNA Cluster Manipulation
Ivan Olonikov, Adrien Le Thomas, and Alexe Aravin
6. Mass Spectrometric Analysis of 3'-End Methylation of piRNAs
Takeo Suzuki, Kenjyo Miyauchi, Yuriko Sakaguchi, and Tsutomu Suzuki
7. HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins
Anastassios Vourekas and Zissimos Mourelatos
8. Analysis of DNA Methylation in Mouse Testes
Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima, and Toru Nakano
9. Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes
Michael Reuter and Ramesh S. Pillai
10. Small RNA Library Construction from Minute Biological Samples
Jessica Matts, Yuliya Sytnikova, Guan-wei Chirn, Gabor Igloi and Nelson Lau
11. Analysis of sDMA Modifications of PIWI Proteins
Shozo Honda, Yoriko Kirino, and Yohei Kirino
12. Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries
Sergey Shpiz and Alla Kalmykova
13. Combined RNA/DNA Fluorescence in situ Hybridization on Whole-Mount Drosophila Ovaries
Sergey Shpiz, Sergey Lavrov, and Alla Kalmykova
14. Fast and Accurate Method to Purify Small Non-Coding RNAs from Drosophila Ovaries
Thomas Grentzinger and Severine Chambeyron
15. Isolation of Zebrafish Gonads for RNA Isolation
Hsin-Yi Huang and Rene Ketting
16. Small RNA Library Construction for High-Throughput Sequencing
Jon McGinn and Ben Czech
17. Analysis of Piwi-Loaded Small RNAs in Tetrahymena
Tomoko Noto, Henriette M. Kurth, and Kazufumi Mochizuki
18. Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs
Hailong Wang, Haidong Huang and Dahua Chen
19. Isolation and Bioinformatics Analyses of Small RNAs Interacting with Germ-Cell Specific Argonaute in Rice
Reina Komiya and Ken-ichi Nonomura
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