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Pichia Protocols
Hardcover
Series: Methods in Molecular Biology, Book 389
ISBN13: 9781588294296
Publisher: Springer Nature
Published: Aug 8 2007
Pages: 268
Weight: 1.20
Height: 0.90 Width: 6.30 Depth: 9.00
Language: English
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).
Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.
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