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Pharmacogenomics: Methods and Protocols

Pharmacogenomics: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 311

Medical Reference

ISBN10: 1588294404
ISBN13: 9781588294401
Publisher: Springer Nature
Published: Aug 15 2005
Pages: 214
Weight: 1.07
Height: 0.79 Width: 6.32 Depth: 9.30
Language: English
Expert researchers and physician/clinicians describe in detail the newest and most commonly used technologies today in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions.

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