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Periodontal Pathogens: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2210
ISBN13: 9781071609385
Publisher: Humana
Published: Aug 20 2020
Pages: 256
Weight: 1.51
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
Part I: Methods for Bacterial Genetic Manipulation
1. Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant
So-ichiro Nishiyama, Yoshiaki Hasegawa, and Keiji Nagano
2. Genetic Manipulations of Oral Spirochete Treponema denticola
Kurni Kurniyati and Chunhao Li
3. Construction of a Gene-Deletion Mutant in Tannerella forsythia
Keiji Nagano and Yoshiaki Hasegawa
4. Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli
Yoshio Kondo
5. Genetic Transformation of Fusobacterium nucleatum
Akihiro Yoshida and Akihiko Ikegami
Part II: Experimental Methods to Examine Virulence Factors
6. Genotyping of Porphyromonas gingivalis in Relationship to Virulence
Atsuo Amano, Youn-Hee Choi, and Hiroki Takeuchi
7. Transport and Polymerization of Porphyromonas gingivalis Type V Pili
Mikio Shoji, Satoshi Shibata, Mariko Naito, and Koji Nakayama
8. Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis
Yoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, and Richard J. Lamont
9. Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis
Thomas Heidler and Karina Persson
10. Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis
Tomoko Kadowaki
11. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis
Dhana G. Gorasia, Eric Hanssen, Paul D. Veith, and Eric C. Reynolds
12. Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis
Keiko Sato
13. Purification of Tannerella forsythia Surface (S-) Layer Proteins
Sreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, and Ashu Sharma
14. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia
Yukitaka Murakami, Keiji Nagano, and Yoshiaki Hasegawa
15. Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model
Satoru Hirayama and Ryoma Nakao
16. Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis
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