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Peptide Antibodies: Methods and Protocols

Peptide Antibodies: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 1348

Medical Reference

ISBN10: 1493949993
ISBN13: 9781493949991
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 366
Weight: 1.46
Height: 0.79 Width: 7.00 Depth: 10.00
Language: English

This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

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Medical Reference