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Protein Folding, Misfolding, and Disease: Methods and Protocols

Protein Folding, Misfolding, and Disease: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 752

BiologyChemistry

ISBN10: 1493956892
ISBN13: 9781493956890
Publisher: Humana
Published: Aug 23 2016
Pages: 249
Weight: 1.01
Height: 0.55 Width: 7.00 Depth: 10.00
Language: English

Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease. In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the analysis of protein folding and misfolding, featuring strategies for expressing and refolding recombinant proteins which can then be utilized in subsequent experiments. This detailed volume also covers methods for analyzing the formation of amyloid, protocols for determining the size and structure of native and misfolded proteins, as well as specific examples of where misfolded proteins can be examined using state-of -the-art technologies. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Hill, Andrew F.

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Biology