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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Production and Characterization of Bacterial Thermostable Cellulase

Production and Characterization of Bacterial Thermostable Cellulase

Kikani, Bhavtosh

Paperback

Medical ReferenceBiology

ISBN10: 3668655634
ISBN13: 9783668655638
Publisher: Grin Verlag
Published: Mar 15 2018
Pages: 44
Weight: 0.15
Height: 0.11 Width: 5.83 Depth: 8.27
Language: English
Project Report from the year 2018 in the subject Biology - Micro- and Molecular Biology, grade: 0, language: English, abstract: Cellulose is one the most abundant carbon sources present on the earth. In the book, the author reported on production optimization and characterization of cellulolytic enzymes by thermophilic bacteria. Various adaptation strategies adapted by thermophilic bacteria are also discussed along with. The thermophilic bacterial strain produced cellulase in the CMC broth, pH 7 containing 0.5% peptone, 0.5% malt extract, 0.2% ammonium sulphate, 0.2% ammonium nitrate and 0.2% NaCl at 50˚C. Interestingly, the bacteria could not grow at 37˚C, confirming its thermophilic nature. Further, the cellulase was characterized after getting partially purified by ammonium sulphate precipitation method. The partially purified cellulase may be employed to hydrolyze agricultural wastes to produce bioethanol, i.e. biofuels. Thus, the present research may help solve issues of crisis of renewable energy as well as environmental pollution.

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