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Polyamines: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 720
ISBN13: 9781493958399
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 523
Weight: 2.04
Height: 1.09 Width: 7.00 Depth: 10.00
Language: English
Part I: Introduction
1. Current Status of the Polyamine Research Field
Anthony E. Pegg and Robert A. Casero, Jr.
Part II: Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels
2. Exploring Polyamine Biosynthetic Diversity through Comparative and Functional Genomics
Anthony J. Michael
3. Characterization of Genes for Polyamine Modulon
Kazuei Igarashi and Keiko Kashiwagi
4. Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines
Lan Xiao and Jian-Ying Wang
5. Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles
Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui
6. Polyamine Block of Inwardly-Rectifying Potassium Channels
Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols
Part III: Transgenic Models for Study of Polyamine Function
7. Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism
David J. Feith
8. Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism
Anne Uimari, Mervi T. Hyvönen, Eija Pirinen, and Leena Alhonen
9. Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine
Xiaojing Wang and Anthony E. Pegg
Part IV: Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification
10. A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease
Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr.
11. Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases
Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis
12. Assay of Deoxyhypusine Synthase Activity
Edith C. Wolff, Seung Bum Lee, and Myung Hee Park
13. Assay of Deoxyhypusine Hydroxylase Activity
Jong Hwan Park, Edith C. Wolff, and Myung Hee Park
Part V: Regulation of Polyamine Content
14. Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase
Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips
15. Protocols for Studying Antizyme Expression and Function
Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji
16. Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family
Chaim Kahana
17. Post-Transcriptional Regulation of Ornithine Decarboxylase
Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz
Part VI: Polyamine Transport and Uptake
18. Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae
Keiko Kashiwagi and Kazuei Igarashi
19. Genetic and Biochemical Analysis of Protozoal Polyamine Transporters
Marie-Pierre Hasne and Buddy Ullman
20. Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake
Johanna Welch, Katrin Svensson, Paulina Kucharzewska, and Mattias Belting
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