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Potencjal produkcji bioetanolu przez bakterie pochodzące z termitów z wykorzystaniem kolb kukurydzy
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ISBN10: 6630250281
ISBN13: 9786630250282
Publisher: Wydawnictwo Nasza Wiedza
Published: Aug 5 2026
Pages: 124
Weight: 0.39
Height: 0.29 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786630250282
Publisher: Wydawnictwo Nasza Wiedza
Published: Aug 5 2026
Pages: 124
Weight: 0.39
Height: 0.29 Width: 6.00 Depth: 9.00
Language: Polish
Celem niniejszego badania byla produkcja bioetanolu w skali laboratoryjnej z kolb kukurydzy z wykorzystaniem bakterii celulolitycznych wyizolowanych z przewodu pokarmowego termitów. Bakterie celulolityczne wyizolowano z dwóch lokalnych gatunków termitów, w tym Coptotermes heimi i Microtermes mycophagous. Kolby kukurydzy poddano procesowi delignifikacji. Po obróbce wstępnej uzyskano dwukrotny spadek zawartości ligniny i 20-procentowy wzrost zawartości celulozy. Spośród sześciu wyizolowanych szczepów bakterii wybrano CH-1 i MYC-1 ze względu na ich potencjalne dzialanie celulolityczne, potwierdzone tworzeniem strefy halo podczas wstępnej selekcji oraz hydrolizą substratu podczas selekcji wtórnej. Dla szczepów CH-1 i MYC-1 zoptymalizowano temperaturę, pH, stężenie substratu, wielkośc inokulum, środki powierzchniowo czynne, jony metali, stężenie PEG oraz aktywnośc enzymów CMCase i FPase. Optymalna wydajnośc bioetanolu wyniosla odpowiednio 5,45 mg/ml i 5,79 mg/ml dla szczepów CH-1 i MYC-1 w procesie jednoczesnej sacharyfikacji i fermentacji. Analiza sekwencji 16S rDNA wykazala homologię z Bacillus cereus (CH-1) i Providencia vermicola (MYC-1). W związku z tym można stwierdzic, że oba izolaty wykazaly potencjal do biokonwersji odpadów rolniczych w cukry fermentowalne, które następnie można poddac fermentacji w celu uzyskania bioetanolu.
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