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Ocena tandemowego nanociala anty-GFP LaG-16-G4S-LaG2
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ISBN10: 6202491280
ISBN13: 9786202491280
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 27 2025
Pages: 68
Weight: 0.23
Height: 0.16 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786202491280
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 27 2025
Pages: 68
Weight: 0.23
Height: 0.16 Width: 6.00 Depth: 9.00
Language: Polish
Nanokorpusy to niekonwencjonalny rodzaj przeciwcial. Znane również jako przeciwciala lańcuchowe ciężkie, występują w surowicy krwi wielblądowatych i nie zawierają polipeptydu lańcucha L, a także brakuje im pierwszej domeny stalej - CH1. Te wlaściwości, a także stosunkowo niewielki rozmiar sprawiają, że są one dobrze przystosowane do uzyskiwania dostępu do miejsc wiązania trudnych celów. Doskonalym przykladem wszechstronnego nanociala jest tandemowe nanocialo LaG-16-G4S-LaG-2, opracowane przez laboratorium Rout, które wykazalo wysoką specyficznośc i powinowactwo do GFP. Silne powinowactwo tandemowego nanociala do tej cząsteczki markerowej pozwala na jego wykorzystanie w oczyszczaniu bialek lub kompleksów bialkowych o niskiej zawartości. W niniejszej pracy przedstawiam eksperymenty mające na celu udostępnienie systemu wychwytywania bialka LaG-16-G4S-LaG-2-GFP i jego późniejszego polączenia z kompleksem wiązania FimGt-DsF do przyszlych badań strukturalnych. Ponadto pokazuję, w jaki sposób system ten można ulepszyc w przyszlości. Jestem przekonany, że przedstawione eksperymenty pomogą wyjaśnic kluczowe etapy nowatorskiego podejścia do oczyszczania bialek i mogą doprowadzic do
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