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Izolacja i molekularne wykrywanie bakteryjnych genów degradacji AHCs
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ISBN10: 6208752450
ISBN13: 9786208752453
Publisher: Wydawnictwo Nasza Wiedza
Published: Mar 17 2025
Pages: 76
Weight: 0.25
Height: 0.18 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786208752453
Publisher: Wydawnictwo Nasza Wiedza
Published: Mar 17 2025
Pages: 76
Weight: 0.25
Height: 0.18 Width: 6.00 Depth: 9.00
Language: Polish
Sześcdziesiąt trzy różne kolonie bakterii tlenowych wyizolowano z próbek ścieków i osadów ściekowych jako degradatory węglowodorów aromatycznych. Cztery izolaty zostaly wybrane jako najsilniejsze izolaty zdolne do wzrostu na podlożach mineralnych uzupelnionych skladnikami BTXHB. Te cztery izolaty zostaly wybrane do identyfikacji, a H12 zostal wybrany do dalszych badań w tym badaniu. Uzyskano charakterystykę morfologiczną i biochemiczną dla czterech izolatów. Do identyfikacji wyizolowanych szczepów wykorzystano amplifikację genu 16S rDNA. Zbadano niektóre warunki hodowli, które wplywają na wzrost i zdolnośc do biodegradacji izolatu bakterii H12. Pseudomonas sp. H12 badano na podlożach mineralnych modyfikowanych skladnikami BTXHB, pojedynczo i w mieszaninie, każdy jako jedyne źródlo węgla w 5 różnych stężeniach i odnotowano różne wydajności usuwania. Eksperymenty minipreparacji plazmidów i utwardzania plazmidów wykazaly, że geny biodegradacji Pseudomonas sp. H12 są pozachromosomalne. Amplifikacja PCR genów katabolicznych BTXHB wykazala, że eksperymentalna bakteria jest nosicielem genów tmoA, xylM, xylA, xylE1.
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