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Pericytes: Methods and Protocols

Pericytes: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2235

Biology

ISBN10: 1071610589
ISBN13: 9781071610589
Publisher: Humana Pr
Published: Feb 13 2022
Pages: 192
Weight: 0.81
Height: 0.44 Width: 7.00 Depth: 10.00
Language: English

This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials ofa cell lineage of increasing interest and therapeutic significance.

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Péault, Bruno M.

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Biology