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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
PCR Cloning Protocols

PCR Cloning Protocols

Chen, Bing-Yuan
Janes, Harry W.

Paperback

Series: Methods in Molecular Biology, Book 192

Medical ReferenceBiology

ISBN10: 1617372811
ISBN13: 9781617372810
Publisher: Springer Nature
Published: Nov 10 2010
Pages: 439
Weight: 1.46
Height: 1.02 Width: 6.00 Depth: 9.00
Language: English
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

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