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Olfactory Receptors: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1003
ISBN13: 9781627033763
Publisher: Humana
Published: Apr 14 2013
Pages: 259
Weight: 1.45
Height: 0.80 Width: 7.00 Depth: 10.00
Language: English
Part I: Knowledge Dissemination of Olfactory Receptors
1. Databases in SenseLab for the Genomics, Proteomics, and Function of Olfactory Receptors
Luis N. Marenco, Gautam Bahl, Lorra Hyland, Jing Shi, Rixin Wang, Peter C. Lai, Perry L. Miller, Gordon M. Shepherd, and Chiquito J. Crasto
2. HORDE: Comprehensive Resource for Olfactory Receptor Genomics
Tsviya Olender, Noam Nativ, and Doron Lancet
3. Identification of Olfactory Receptor Genes from Mammalian Genome Sequences
Yoshihito Niimura
Part II: Theoretical Assessments of Olfactory Receptor Structure and Function
4. Molecular Modelling of Odorant / Olfactory Receptor Complexes
Landry Charlier, Jérémie Topin, Peter C. Lai, Chiquito J. Crasto, and Jerome Golebiowski
5. The Electrical Properties of Olfactory Receptors in the Development of Biological Smell Sensors
Eleanora Alfinito, Jeremy Pousset, and Lino Reggiani
6. A Hit Map-Based Statistical Method to Predict Best Ligands for Orphan Olfactory Receptors: Natural Key Odorants Versus 'Lock Picks'
Dietmar Krautwurst and Matthias Kotthoff
Part III: Expression Systems for Olfactory Receptors
7. A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems
Karolina Corin, Brian Cook, and Shuguang Zhang
8. Human Olfactory Receptors: Recombinant Expression in the Baculovirus / Sf9 Insect Cell System, Functional Characterization, and Odorant Identification
Valéry Matarazzo and Catherine Ronin
9. Single Cell RT-PCR Identification of Odorant Receptors Expressed by Olfactory Neurons
Bettina Malnic
10. Next-Generation Sequencing of the Human Olfactory Receptors
Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, and Nicholas Katsanis
11. Deciphering Activation of Olfactory Receptors Using Heterologous Expression in Saccharomyces cerevisiae and Bioluminescence Resonance Energy Transfer (BRET)
Guenhaël Sanz and Edith Pajot-Augy
12. Olfactory Receptors in Human Airway Epithelia
Xiaoling Gu and Yehuda Ben-Shahar
Part IV: Functional Analysis of Olfactory Receptors
13. Functional Characterization of Insect Olfactory Receptor Neurons Through In Vivo Approaches
Michel Renou and Philippe Lucas
14. Functional Assay of Mammalian and Insect Olfactory Receptors Using Xenopus Oocytes
Charles W. Luetje, Andrew S. Nichols, Ana Castro, and Benjamin L. Sherman
15. Pharmacology of Mammalian Olfactory Receptors
Richard S. Smith, Zita Peterlin, and Ricardo C. Araneda
16. Visualizing Olfactory Receptor Expression and Localization in Drosophila
Michael Saina and Richard Benton
17. Deorphanization of Human Olfactory Receptors by Luciferase and Ca-Imaging Methods
Kaveh A. Baghaei
Part V: Olfactory Receptor Evolution
18. Evolution of Olfactory Receptors
Kara C. Hoover
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