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Nuclear Reprogramming: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1222
ISBN13: 9781493954414
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 285
Weight: 1.15
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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