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Nf-κb Transcription Factors: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2366
ISBN13: 9781071616710
Publisher: Humana Pr
Published: Jul 9 2022
Pages: 359
Weight: 1.43
Height: 0.78 Width: 7.00 Depth: 10.00
Language: English
Part I: General Methods for Analyzing NF-κB Activation
1. Measuring NF-kB Phosphorylation and Acetylation
Nikita Tushar Modi and Lin-Feng Chen
2. Biochemical Methods to Analyze the Subcellular Localization of NF- κB Proteins Using Cell Fractionation
Davide Vecchiotti, Daniela Verzella, Daria Capece, Mauro Di Vito Nolfi, Barbara Di Francesco, Jessica Cornice, Guido Franzoso, Edoardo Alesse, and Francesca Zazzeroni
3. Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proteins in Human Tissues
Davide Vecchiotti, Daniela Verzella, Daria Capece, Jessica Cornice, Mauro Di Vito Nolfi, Barbara Di Francesco, Guido Franzoso, Edoardo Alesse, and Francesca Zazzeroni
4. High-Throughput Analysis of the Cell- and DNA Site-Specific Binding of Native NF-κB Dimers Using Nuclear Extract Protein-Binding Microarrays (NextPBMs)
Heather Hook, Rose Zhao, David Bray, Jessica L. Keenan, and Trevor Siggers
5. Molecular and Biochemical Approaches to Study the Evolution of NF-κB Signaling in Basal Metazoans
Pablo J. Aguirre Carrión, Leah M. Williams, and Thomas D. Gilmore
Part II: Methods for Studying the Activation of NF-κB Downstream of Distinct Signaling Pathways
6. Methods for Modulating the Pathway of NF-kB Using Short Hairpin RNA (ShRNA)
Marta Moretti, Barbara Di Francesco, Mauro Di Vito Nolfi, Annapaola Angrisani, and Enrico De Smaele
7. Immunoblot Analysis of the Regulation of TNF Receptor Family-Induced NF-kB Signaling by c-IAP Proteins
Eugene Varfolomeev, Tatiana Goncharov, and Domagoj Vucic
8. Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-kB Activation in Jurkat T-Cells
Andreas Gewies, Carina Gra , and Daniel Krappmann
9. Analysis of Calcium Control of Canonical NF-κB Signaling in B Lymphocytes
Corbett T. Berry, Michael J. May, and Bruce D. Freedman
10. A Kinase Assay for Measuring the Activity of the NIK-IKK1 Complex Induced via the Noncanonical NF-κB Pathway
Tapas Mukherjee, Yashika Ratra, Balaji Banoth, Alvina Deka, Smarajit Polley, and Soumen Basak
11. Analyze the SUMOylation of IKKγ/NEMO During Genotoxic Stress
Zhao-Hui Wu and Shigeki Miyamoto
Part III: Methods for Analyzing NF-κB Activation in Physiology and Disease
12. Analysis of the Contribution of NF-kB in the Regulation of Chemotherapy-Induced Cell Senescence by Establishing a Tetracycline-Regulated Cell System
Francesco Pacifico, Elvira Crescenzi, and Antonio Leonardi
13. Methods to Detect NF-κB Activity in Tumor-Associated Macrophage (TAM)
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