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Natural Killer (Nk) Cells: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2463
ISBN13: 9781071621592
Publisher: Humana Pr
Published: Mar 29 2022
Pages: 330
Weight: 1.79
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
Part I: NK Cell Specification
1. Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria
Ao Mei, Elaheh Hashemi, Mohamed Khalil, Dandan Wang, and Subramaniam Malarkannan
2. Purification of Primary Decidual Natural Killer Cells for Functional Analysis
Ângela C. Crespo, Aria Alexander, and Tamara Tilburgs
3. In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells
Ines Ullmo, Nahide Koksal, Heather Y.K. Ang, and Hugh J.M. Brady
4. Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells
Akira Niwa and Megumu K. Saito
5. Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease
Liang Shan, Richard A. Flavell, and Dietmar Herndler-Brandstetter
6. Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics' Single-Cell RNA-Seq
Dominic Paul Lee, Wang Jiehao Ray, Tan Pee Mei, Shawn Hoon, Jonathan Scolnick, and Gene W. Yeo
7. Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses
Dandan Wang, Robert Burns, Mohamed Khalil, Ao Mei, Elaheh Hashemi, and Subramaniam Malarkannan
8. Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells
Elaheh Hashemi, Ao Mei, Dandan Wang, Mohamed Khalil, and Subramaniam Malarkannan
9. Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes
Simon Grassmann, Joseph C. Sun, and Veit R. Buchholz
10. Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis
Amera L. Martinez, Michael J. Shannon, Shira E. Eisman, Everardo Hegewisch-Solloa, Aneeza N. Asif, Tasneem A.M. Ebrahim, and Emily M. Mace
11. Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling
Noriko Sato, Lawrence P. Szajek, and Peter L. Choyke
Part II: NK Cell Functional Assays
12. Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer
Javier Traba and Olga M. Antón
13. Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors
Lizeth G. Meza Guzman and Sandra E. Nicholson
14. Method to Study Adaptive NK Cells Following MCMV Infections
Mohamed Khalil, Ao Mei, Elaheh Hashemi, Dandan Wang, Megan Schumacher, Scott Terhune, and Subramaniam Malarkannan
15. Assessing the Response of Human NK Cell Subsets to Infec
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