Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
612-822-4611

  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
A New dot-ELISA Kit

A New dot-ELISA Kit

Alam, Jehan
Hasan, Najmul

Paperback

Biology

ISBN10: 3659403083
ISBN13: 9783659403088
Publisher: Lap Lambert Academic Pub
Published: May 31 2013
Pages: 120
Weight: 0.41
Height: 0.28 Width: 6.00 Depth: 9.00
Language: English
Poultry farming is the most dynamic & 2nd major industry in Pakistan. It has challenges of outbreaks of viral diseases resulting in high mortality, morbidity and huge economic loss in Pakistan. Conventional diagnostic tests are expensive, laborious & require technical expertise. Hence, a highly sensitive & specific dot-ELISA kit is developed, in two steps; first whole antigen is prepared from AIV, NDV and IBV and is applied onto nitrocellulose membrane; second rabbit anti-chicken antibodies is raised, purified and conjugated with enzyme HRP. Dot ELISA results are much comparative with ELISA & have advantages of high specificity, sensitivity & facile. This kit has the advantage of loading multiple antigens from local isolates & can be performed even at poultry forms; with no technical expertise or advance laboratory facilities for interpretations. Even the staff can test their bird's immune response after vaccination or test for the presence of infection. It can be prepared & used on large scale; this combine approach of several antigens on single strip play a vital role in the diagnosis of poultry diseases or determining immune status and help in controlling diseases in Pakistan.

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