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Neurodegeneration: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 793
ISBN13: 9781493962204
Publisher: Humana
Published: Aug 23 2016
Pages: 512
Weight: 2.02
Height: 1.08 Width: 7.00 Depth: 10.00
Language: English
Part I. Neurodegeneration
1. Introduction to Neurodegenerative Diseases and Related Techniques
Hibiki Kawamata and Giovanni Manfredi
2. In vivo and In vitro Determination of Cell Death Markers in Neurons
Nicole Naniche, Daniela Sua, and Piera Pasinelli
3. Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods
Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, and Joseph P. Pierce
Part II. Neural Cell Culture Techniques
4. Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex
Hyun-Jeong Kim and Jordi Magrané
5. Isolation and Culture of Postnatal Spinal Motoneurons
Carol Milligan and David Gifondorwa
6. Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration
Stuart M. Chambers, Yvonne Mica, Lorenz Studer, and Mark J. Tomishima
7. Neural Differentiation of Induced Pluripotent Stem Cells
Mark Denham and Mirella Dottori
Part III. Models of Neurodegeneration
8. Developing Yeast Models of Human Neurodegenerative Disorders
Alejandro Ocampo and Antoni Barrientos
9. Modeling Dopamine Neuron Degeneration in C. elegans
Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, and Kim A. Caldwell
10. Role of the Proteasome in Fly Models of Neurodegeneration
Chun-Hung Yeh, Marlon Jansen, and Thomas Schmidt-Glenewinkel
11. Modeling Neurodegenerative Diseases in Zebrafish Embryos
Angela Laird and Wim Robberecht
12. Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases
Kindiya Geghman and Chenjian Li
13. Modeling Focal Cerebral Ischemia In vivo
Katherine Jackman, Alexander Kunz, and Costantino Iadecola
14. Pharmacological Models of Parkison's Disease in Rodents
Peter Klivenyi and Laszlo Vecsei
15. Behavioral Phenotyping of Mouse Models of Neurodegeneration
Magali Dumont
Part IV. Neurodegenerative Disease Mechanisms - Proteinopathies
16. Characterization of Amyloid Deposits in Neurodegenerative Diseases
Ruben Vidal and Bernardino Ghetti
17. Detection of Protein Aggregates in Neurodegenerative Diseases
Han-xiang Deng, Eileen H. Bigio, and Teepu Siddique
18. Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures
Natura Myeku, Maria Jose Metcalfe, Qian Huang, and Maria Figueiredo-Pereira
Part V. Neurodegenerative Disease Mechanisms - Mitochondria
19. Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium
Andrey Y. Abramov and Michael R. Duchen
20. Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue
Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, and Anatoly A. Starkov
21. Monitoring Mitophagy in Neuronal Cell Cultures
Jianhui Zhu, Ruben K. Dagda, and Charleen T. Chu
Part VI. Neurodegenerative Disease Mechanisms - Biological functions
22. Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging
Davide Tampellini and Gunnar K. Gouras
23. Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices
Doo Yeon Kim and Dora M. Kovacs
24. Imaging Pr
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