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Mouse Oocyte Development: Methods and Protocols

Mouse Oocyte Development: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1818

Biology

ISBN10: 1493986023
ISBN13: 9781493986026
Publisher: Springer Nature
Published: Jul 1 2018
Pages: 174
Weight: 1.20
Height: 0.50 Width: 7.00 Depth: 10.00
Language: English

This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Biology