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Microsatellites: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1006
ISBN13: 9781627033886
Publisher: Springer Nature
Published: Apr 2 2013
Pages: 339
Weight: 1.75
Height: 1.00 Width: 6.90 Depth: 10.10
Language: English
1. Microsatellites: Evolution and Contribution
Panagiotis Madesis, Ioannis Ganopoulos, and Athanasios Tsaftaris
Part I: Discovery and Development of Microsatellites
2. Screening of Genomic Libraries
Valdenice M. Novelli, Mariângela Cristofani-Yaly, Marinês Bastianel, Dario A. Palmieri, and Marcos A. Machado
3. PCR-Based Identification of Microsatellite Arrays (PIMA)
Heng-Sheng Lin and Song-Bin Chang
4. Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO)
Kaisa Rikalainen
5. Microsatellite DNA Capture from Enriched Libraries
Elena G. Gonzalez and Rafael Zardoya
6. Next-Generation Sequencing for High-Throughput Molecular Ecology: A Step-by-Step Protocol for Targeted Multilocus Genotyping by Pyrosequencing
Jonathan B. Puritz and Robert J. Toonen
7. Optimizing Selection of Microsatellite Loci from 454 Pyrosequencing Via Post-Sequencing Bioinformatic Analyses
Iria Fernandez-Silva and Robert J. Toonen
8. Identification of DNA-Microsatellite Markers for the Characterization of Somatic Embryos in Quercus suber
Arancha Gómez-Garay, Ma Ángeles Bueno, and Beatriz Pintos
Part II: Amplification and Visualization
9. Simple Sequence Repeats (SSR) Amplification
Kundapura V. Ravishankar and Bommisetty Padmakar
10. Microsatellite Amplification in Plants: Optimization Procedure of Major PCR Components
Sana Ghaffari and Nejib Hasnaoui
11. Development of a Multiplex PCR Assay for Characterization of Embryonic Stem Cells
Rajarshi Pal, Murali Krishna Mamidi, Anjan Kumar Das, Mahendra Rao, and Ramesh Bhonde
12. Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis for Visualization of Single Sequence Repeats
James Anderson, Drew Wright, and Khalid Meksem
Part III: Automated Capillary Sequencers
13. Microsatellite Fragment Analysis Using the ABI Prism(R) 377 DNA Sequencer
Mark A. Renshaw, Melissa Giresi, and J. Adams
14. Robust and Inexpensive SSR Markers Analyses Using LI-COR DNA Analyzer
Maria del Rosario Herrera and Marc Ghislain
15. The Use of the MegaBACE for Sequencing and Genotype Analysis
Pamela A. Burger
16. Analyzing Microsatellites Using the QIAxcel System
Deborah A. Dean, Phillip A. Wadl, Denita Hadziabdic, Xinwang Wang, and Robert N. Trigiano
Part IV: Scoring and Data Analysis
17. Microsatellite Analysis of Malaria Parasites
Pamela Orjuela-Sánchez, Michelle C.C. Brandi, and Marcelo U. Ferreira
18. Informativeness of Microsatellite Markers
M. Humberto Reyes-Valdés
19. Microsatellite Data Analysis for Population Genetics
Kyung Seok Kim and Thomas W. Sappington
20. Molecular Mapping and Breeding with Microsatellite Markers
David A. Lightfoot and Muhammad J. Iqbal
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