Sign-In
Cart
Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition
Paperback
Please select the version of book you would like to purchase.
ISBN10: 1936113015
ISBN13: 9781936113019
Publisher: Cold Spring Harbor Laboratory Press
Published: Oct 31 2013
Pages: 814
Weight: 5.20
Height: 1.70 Width: 8.50 Depth: 10.90
Language: English
ISBN13: 9781936113019
Publisher: Cold Spring Harbor Laboratory Press
Published: Oct 31 2013
Pages: 814
Weight: 5.20
Height: 1.70 Width: 8.50 Depth: 10.90
Language: English
This 4th edition of the Mouse Manual-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of
this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable
this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable
direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and
rewriting this fourth edition to include new information and protocols on: - generation of induced pluripotent stem cells
- RNA microinjections
- lentiviral microinjections and infection
- assisted reproduction techniques for sperm and embryo cryopreservation
- isolation, generation, and transplantation of spermatogonial stem cell lines
- in utero electroporation of gene constructs into postimplantation embryos
- vibratome sectioning of live and fixed tissues for imaging thick tissue sections
- whole-mount fluorescent staining methods for three-dimensional visualization In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an
outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since
then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice. I have the third edition. Why should I buy the fourth edition?
The new technologies regarding induced pluripotent stem cells and RNA injection and CRISPR technology are changing the field and opening up new avenues of investigation. The protocols for these as well as other up-to-the minute techniques in the fourth edition are by the leading proponents in the
field and offer clear step-by-step guidance with copious illustrations. To whom is this edition addressed-the neophyte or the experienced investigator?
Both groups can benefit from this manual. For the neophyte, the summary of mouse development and the how-to's of setting up a working laboratory are an invaluable introduction to the field. For the experienced investigator, the detailed protocols of new techniques in the field, with extensive
troubleshooting and over-the-shoulder advice, allow easy integration of these techniques into a laboratory's research objectives.
Are the protocols in this book better than those available for download on the Web?
Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they
work. And they include the context and troubleshooting information that many online protocols do not have. In the case of the protocols in this manual, there is a bit of art required in the performance of these protocols. But the experience and advice of these authors provides all of the
information needed to achieve success in the lab.
rewriting this fourth edition to include new information and protocols on: - generation of induced pluripotent stem cells
- RNA microinjections
- lentiviral microinjections and infection
- assisted reproduction techniques for sperm and embryo cryopreservation
- isolation, generation, and transplantation of spermatogonial stem cell lines
- in utero electroporation of gene constructs into postimplantation embryos
- vibratome sectioning of live and fixed tissues for imaging thick tissue sections
- whole-mount fluorescent staining methods for three-dimensional visualization In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an
outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since
then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice. I have the third edition. Why should I buy the fourth edition?
The new technologies regarding induced pluripotent stem cells and RNA injection and CRISPR technology are changing the field and opening up new avenues of investigation. The protocols for these as well as other up-to-the minute techniques in the fourth edition are by the leading proponents in the
field and offer clear step-by-step guidance with copious illustrations. To whom is this edition addressed-the neophyte or the experienced investigator?
Both groups can benefit from this manual. For the neophyte, the summary of mouse development and the how-to's of setting up a working laboratory are an invaluable introduction to the field. For the experienced investigator, the detailed protocols of new techniques in the field, with extensive
troubleshooting and over-the-shoulder advice, allow easy integration of these techniques into a laboratory's research objectives.
Are the protocols in this book better than those available for download on the Web?
Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they
work. And they include the context and troubleshooting information that many online protocols do not have. In the case of the protocols in this manual, there is a bit of art required in the performance of these protocols. But the experience and advice of these authors provides all of the
information needed to achieve success in the lab.
Also in
Biology
The Age of Living Machines: How Biology Will Build the Next Technology Revolution
Hockfield, Susan
Paperback
$17.05
Entangled Life: How Fungi Make Our Worlds, Change Our Minds & Shape Our Futures
Sheldrake, Merlin
Paperback
$18.00
History by a Hair: Thomas Jefferson, Sally Hemings & Solving a 200-Year-Old Mystery
Kurin, Richard
Hardcover
$31.50
Hidden Creatures: Luscious Leeches, Bashful Botflies, and the Wondrous, History-Shaping World of Parasites
Martins, Dino
Hardcover
$27.00
The World Beneath Our Feet: The Hidden Life of Soil and Why It Matters to Us All
Ashwood, Frank
Hardcover
$27.00
Beyond Inheritance: Our Ever-Mutating Cells and a New Understanding of Health
Khamsi, Roxanne
Hardcover
$28.50
National Geographic the Photo Ark: One Man's Quest to Document the World's Animals
Chadwick, Douglas H.
Sartore, Joel
Hardcover
$19.99
Togetherness: Symbiosis and the Hidden Story of Life's Greatest Collaborations
Hooper, Rowan
Hardcover
$31.50
Go Forth and Forage: A Guide to Foraging Over 50 of the Most Common Edible & Medicinal North American Mushrooms
Johnson, Whitney
Paperback
$23.74
The Biology of Trauma: How the Body Holds Fear, Pain, and Overwhelm, and How to Heal It
Apigian, Aimie
Hardcover
$26.96
The Power of Life: The Invention of Biology and the Revolutionary Science of Jean-Baptiste Lamarck
Riskin, Jessica
Hardcover
$30.40
Cells at Work! Picture Book 1: The Great Bacteria vs. White Blood Cell Battle!
Makimura, Kumi
Hardcover
$13.29
Princeton Review AP Biology Premium Prep, 29th Edition: 6 Practice Tests + Digital Practice Online + Content Review
The Princeton Review
Paperback
$26.59
Ernst Haeckel's Art Forms in Nature: A Visual Masterpiece of the Natural World
Haeckel, Ernst
Hardcover
$48.74
Fungi Decoded: Adventures in the Mycosphere--Encounters with Thirty Fungi
Bunyard, Britt A.
Paperback
$18.99
The Whole Truth: The Hidden Agendas Behind Decades of Health Misinformation--And What to Do about It
Campbell, T. Colin
Campbell, Nelson
Hardcover
$26.55
Peterson Field Guide to Mushrooms of North America, Second Edition
Rohrer, Joseph R.
McKnight Ward, Kirsten
McKnight, Karl B.
Paperback
$24.69
Night Magic: Adventures Among Glowworms, Moon Gardens, and Other Marvels of the Dark
Henion, Leigh Ann
Hardcover
$28.50
Forbidden Facts: Government Deceit & Suppression about Brain Damage from Childhood Vaccines
de Becker, Gavin
Hardcover
$31.34
Bioinspired: How Nature Made Us Modern, Drives Today's Technological Innovation, and Inspires a Future Worth Inventing
Stier, Samuel Cord
Hardcover
$33.25
Food Intelligence: The Science of How Food Both Nourishes and Harms Us
Hall, Kevin, PhD
Belluz, Julia
Hardcover
$28.50
All That Remains: A Renowned Forensic Scientist on Death, Mortality, and Solving Crimes
Black, Sue
Paperback
$18.04
Psilocybin: Magic Mushroom Grower's Guide: A Handbook for Psilocybin Enthusiasts
Oss, O. T.
Oeric, O. N.
Paperback
$18.00
A Year with the Seals: Unlocking the Secrets of the Sea's Most Charismatic and Controversial Creatures
Morris, Alix
Hardcover
$28.50
VAX Facts: What to Consider Before Vaccinating at All Ages & Stages of Life
Hoover, Deedee
Thomas, Paul
Paperback
$25.60
The Big One: How We Must Prepare for Future Deadly Pandemics
Olshaker, Mark
Osterholm, Michael T.
Hardcover
From $19.99
The Aesthetic Revolution: Embracing Vanity and the Future of Fitness to Unlock Your Healthiest Self
Israetel, Mike
Hardcover
$28.50
Mushrooms of the Redwood Coast: A Comprehensive Guide to the Fungi of Coastal Northern California
Siegel, Noah
Schwarz, Christian
Paperback
$33.25
The Gut-Brain Paradox: Improve Your Mood, Clear Brain Fog, and Reverse Disease by Healing Your Microbiome
Gundry MD, Steven R.
Hardcover
$30.40
Fungi of Temperate Europe: Keys to the Basidiomycotes
Frøslev, Tobias G.
Læssøe, Thomas
Petersen, Jens H.
Hardcover
$90.25
True Hallucinations: Being an Account of the Author's Extraordinary Adventures in the Devil's Paradis
McKenna, Terence
Paperback
$17.09
Psilocybin Mushrooms: A Comprehensive Guide to Growing and Using Psychedelic Mushrooms
Madden, Michael
Paperback
$14.24
The Art and Science of Foodpairing: 10,000 Flavour Matches That Will Transform the Way You Eat
Coucquyt, Peter
Lahousse, Bernard
Langenbick, Johan
Hardcover
$47.45
Entangled Life: How Fungi Make Our Worlds, Change Our Minds & Shape Our Futures
Sheldrake, Merlin
Hardcover
$28.50
The Little Book of Mushrooms: An Illustrated Guide to the Extraordinary Power of Mushrooms
Dorr, Alex
Hardcover
From $11.99
Regents Exams and Answers: Life Science: Biology, Second Edition
Barron's Educational Series
Paperback
$18.04
Mushrooming: An Illustrated Guide to the Fantastic, Delicious, Deadly, and Strange World of Fungi
Borsato, Diane
Hardcover
$23.70
The Contagion Myth: Why Viruses (Including Coronavirus) Are Not the Cause of Disease
Cowan, Thomas S.
Fallon Morell, Sally
Hardcover
$31.34
From These Lands: Sharing Our Natural and Cultural Heritage
National Museum of Natural History
Hardcover
$18.95
Mushrooms of Cascadia, Second Edition: An Illustrated Key to the Fungi of the Pacific Northwest
Beug, Michael
Paperback
$26.60
Human Errors: A Panorama of Our Glitches, from Pointless Bones to Broken Genes
Lents, Nathan H.
Paperback
$17.09
How to Win Friends and Influence Fungi: Collected Quirks of Science, Tech, Engineering, and Math from Nerd Nite
Balakrishnan, Chris
Wasowski, Matt
Paperback
$19.00
The Mushroom Hunters: A Hidden World of Food, Money, and (Mostly Legal) Adventure
Cook, Langdon
Paperback
$19.95
Superconvergence: How the Genetics, Biotech, and AI Revolutions Will Transform Our Lives, Work, and World
Metzl, Jamie
Paperback
$20.89
The Life Machines: How Taking Care of Your Mitochondria Can Transform Your Health
Mochly-Rosen, Daria
Rosen, Emanuel
Hardcover
$27.55
The Ocean's Menagerie: How Earth's Strangest Creatures Reshape the Rules of Life
Harvell, Drew
Hardcover
$28.80
Field Notes from a Fungi Forager: An Illustrated Journey Through the World of Pacific Northwest Mushrooms
Rodriguez, Ashley
Hardcover
$21.80
The Mycocultural Revolution: Transforming Our World with Mushrooms, Lichens, and Other Fungi
McCoy, Peter
Hardcover
$17.05
Field Guide to Wild Mushrooms of Pennsylvania and the Mid-Atlantic: Revised and Expanded Edition
Russell, Bill
Paperback
$23.70
Gut Check: Unleash the Power of Your Microbiome to Reverse Disease and Transform Your Mental, Physical, and Emotional Health
Gundry MD, Steven R.
Hardcover
$30.40
National Audubon Society Wildflowers of North America: The Complete Identification Reference to Wildflowers--With Full-Color Photographs; Updated Rang
National Audubon Society
Hardcover
$37.95
Life in Sync: The Science of Internal Clocks and How We're Disrupting Them
Gander, Philippa
Hardcover
$21.80
The Serengeti Rules: The Quest to Discover How Life Works and Why It Matters
Carroll, Sean B.
Paperback
$18.00
The Pale Blue Data Point: An Earth-Based Perspective on the Search for Alien Life
Willis, Jon
Hardcover
$24.70
Pull: How Gravity Shapes Your Body, Steadies the Mind, and Guides Our Health
Spiegel, Brennan
Hardcover
$28.50
Sea Wonders: The Octopus, the Cuttlefish, and the Squid
Tomasinelli, Francesco
Colombo, Marco
Hardcover
$18.95
The Double Helix: A Personal Account of the Discovery of the Structure of DNA
Watson, James D.
Paperback
$18.99
The Hidden Half of Nature: The Microbial Roots of Life and Health
Montgomery, David R.
Biklé, Anne
Paperback
From $10.99