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Mammary Gland Development: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1501
ISBN13: 9781493982127
Publisher: Humana
Published: Jul 10 2018
Pages: 358
Weight: 1.42
Height: 0.77 Width: 7.00 Depth: 10.00
Language: English
1. Overview of the Development of the Mouse Mammary Gland; A Comparison of Mammary Gland Development in Mouse and Humans
Torsten Stein and Sara McNally
Part I: In Vivo Models
2. Prenatal Mammary Gland Development in the Mouse - Research Models and Techniques for its Study from Past to Present
Jaqueline Veltmaat
3. Pubertal Mammary Gland Development: Elucidation Of In Vivo Morphogenesis Using Murine Models
Jean Mcbryan and Jillian Howlin
4. Analysis of Mammary Gland Phenotypes by Transplantation of the Genetically Marked Mammary Epithelium
Duje Buric and Cathrin Brisken
5. Pubertal Ductal Morphogenesis: Isolation and Transcriptome Analysis of the Terminal End Bud
Joanna S. Morris and Torsten Stein
6. RNA Profiling of Non-Cultured Fibroblasts Isolated from Pubertal Mouse Mammary Gland Sections
Ayman M. Ibrahim, Claire Cairney, Joanna S. Morris, and Torsten Stein
7. Analysis of the Involuting Mouse Mammary Gland: An In Vivo Model for Cell Death
Bethan Lloyd-Lewis, Timothy J. Sargeant, Peter A. Kreuzaler, Henrike K. Resemann, Sara Pensa, and Christine J. Watson
Part II: In Vitro 2D & 3DModels
8. Contractility Assay for Established Myoepithelial Cell Lines
Stéphanie Cagnet, Marina A. Glukhova, and Karine Raymond
9. The use of 3D Culture of Primary Mammary Epithelial Cells to Define Molecular Entities Required for Acinus Formation. Analysing MAP Kinase Phosphatases
Malgorzata Gajewska and Sara Mcnally
10. A 3D Fibroblast-Epithelium Co-culture Model for Understanding Micro-Environmental Role in Branching Morphogenesis of the Mammary Gland
Zuzana Koledova and Pengfei Lu
11. RNA Sequencing Analysis Reveals Expression of a Transient EMT Profile During the Early During Organization of MCF10A Cells in 3D
Benedikt Minke, Finian Martin, Walter Kolch, and Drieke Vandamme
12. A 3D Culture Model to Study How Fluid Pressure and Flow Affect the Behavior of Aggregates of Epithelial Cells
Alexandra S. Piotrowski, Allison K. Simi, Mei Fong Pang, Joe Tien, and Celeste M. Nelson
Part III: MAMMARY STEM CELLS
13. Purification of Distinct Subsets of Epithelial Cells from Normal Human Breast Tissue
Mona Shehata and John Stingl
14. Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate
Gilbert Smith and Corrine A. Boulanger
15. Lineage Tracing of Mammary Stem and Progenitor Cells
Anoeska A.A. Van De Moosdijk, Nai Yang Fu, Anne C. Rios, Jane E. Visvader, and Renée Van Amerongen
Part IV: TRANSLATIONAL APPLICATIONS
16. Assessment of Novel Proteins by Tissue Microarray Technology
Laoighse Mulrane, William M. Galla
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