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Mammalian Cell Viability: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 740
ISBN13: 9781493961535
Publisher: Humana
Published: Aug 23 2016
Pages: 240
Weight: 0.98
Height: 0.53 Width: 7.00 Depth: 10.00
Language: English
1. Cell Viability Assays: Introduction
Martin J. Stoddart
2. Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods
Kristine S. Louis and Andre C. Siegel
3. Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay
László Kupcsik
4. WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells
Martin J. Stoddart
5. Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye
Ewa M. Czekanska
6. The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability
Ning Ke, Xiaobo Wang, Xiao Xu, and Yama A. Abassi
7. Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays
Caroline Woolston and Stewart Martin
8. Annexin V / 7-AAD Staining in Keratinocytes
Maya Zimmermann and Norbert Meyer
9. Measurement of Caspase Activity: From Cell Populations to Individual Cells
Gabriela Paroni and Claudio Brancolini
10. Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes
Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz
11. Multiplexing Cell Viability Assays
Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar
12. Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death
Terry L. Riss, Richard A. Moravec, and Andrew L. Niles
13. Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs
Heather M. Powell, Alexis D. Armour, and Steven T. Boyce
14. Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers
Benjamin Gantenbein-Ritter, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, and Sibylle Grad
15. Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections
Katharina Jähn and Martin J. Stoddart
16. Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay
Won-Kyu Ju and Keun-Young Kim
17. Exploiting the Liberation of Zn2+ to Measure Cell Viability
Christian J. Stork and Yang V. Li
18. Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs
Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou
19. Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test
Sophie Verrier, Alina Zoladek, and Ioan Notingher
20. Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses
Olivier Braissant and Alma U. Dan Daniels
21. Digital Image Processing of Live/Dead Staining
Pieter Spaepen, Sebastian De Boodt, Jean-Marie Aerts, and Jos Vander Sloten
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