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Myogenesis: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 798
ISBN13: 9781617793424
Publisher: Springer Nature
Published: Nov 1 2011
Pages: 558
Weight: 2.55
Height: 1.50 Width: 7.00 Depth: 10.20
Language: English
Part I: Isolation and Analysis of Skeletal Muscle Precursor Cells
1. Isolation and Characterization of Human Fetal Myoblasts
Ariya D. Lapan and Emanuela Gussoni
2. Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System
Maria Elena Danoviz and Zipora Yablonka-Reuveni
3. Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting (FACS) Cytometry
Alessandra Pasut, Paul Oleynik, and Michael A. Rudnicki
4. Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles
Mattia Quattrocelli, Giacomo Palazzolo, Ilaria Perini, Stefania Crippa, Marco Cassano, and Maurilio Sampaolesi
5. Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control
Eric J. Cavanaugh, Jennifer R. Crew, and Joseph X. DiMario
6. Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies
Judy E. Anderson, Ashley C. Wozniak, and Wataru Mizunoya
7. Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm
Sara J. Venters and Charles P. Ordahl
Part II: Non-Mammalian Models of Myogenesis
8. Analysis of Skeletal Muscle Development in Drosophila
Ginny R. Morriss, Anton L. Bryantsev, Maria Chechenova, Elisa M. LaBeau, TyAnna L. Lovato, Kathryn M. Ryan, and Richard M. Cripps
9. Immunocytochemistry to Study Myogenesis in Zebrafish
Nathan C. Bird, Stefanie E. Windner, and Stephen H. Devoto
10. Immunofluorescent Localization of Proteins in C. elegans Muscle
Kristy J. Wilson, Hiroshi Qadota, and Guy M. Benian
Part III: Experimental Models and Analysis of Skeletal Muscle Exercise and Disuse
11. Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle
Stephen E. Alway and Robert G. Cutlip
12. Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading
Sue C. Bodine and Keith Baar
13. Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size
Chia-Ling Wu and Susan C. Kandarian
14. In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique
Marta L. Fiorotto, Horacio A. Sosa, Jr., and Teresa A. Davis
Part IV: Generation of Viral Vectors and Transgenic Mice
15. Recombinant Adeno-Associated Viral Vector Production and Purification
Jin-Hong Shin, Yongping Yue, and Dongsheng Duan
16. Generation of Lentiviral Vectors for Use in Skeletal Muscle Research
Christophe Pichavant and Jacques P. Tremblay
17. Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice
Christoph Lepper and Chen-Ming Fan
Part V: Muscle Profiling
18. Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay
Shephali Bhatnagar, Siva K. Panguluri, and Ashok Kumar
19. Analysis of Lipid Profiles in Skeletal Muscles
Vassilis Mougios and Anatoli Petridou
20. Proteomic Analysis of Dystrophic Muscle
Caroline Lewis, Philip Doran, and Kay Ohlendieck
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