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Mycobacteria Protocols
Paperback
Series: Methods in Molecular Biology, Book 2314
ISBN13: 9781071614624
Publisher: Humana
Published: Jul 8 2022
Pages: 736
Weight: 2.83
Height: 1.50 Width: 7.00 Depth: 10.00
Language: English
1. Culturing Mycobacteria
Elizabeth Wallace, Debra Hendrickson, Nicholas Tolli, Carolina Mehaffy, MarÃa Peña, Jerry A. Nick, Phillip Knabenbaur, Jackson Watkins, Anne Simpson, Anita G. Amin, Delphi Chatterjee, Karen M. Dobos, Ramanuj Lahiri, Linda Adams, Michael Strong; Max Salfinger, Rebecca Bradford, Timothy T. Stedman, Marco A. Riojas, and Manzour Hernando Hazbón
2. DNA Isolation from Mycobacteria
Heena Jagatia and Daire Cantillon
3. Extraction and Separation of Mycobacterial Proteins
Megan Lucas, Joan M. Ryan, Jackson Watkins, Kala Early, Nicole A. Kruh-Garcia, Carolina Mehaffy, and Karen M. Dobos
4. Lipid and Lipoarabinomannan Isolation and Characterization
Marie-Antoinette Lanéelle, Lucie Spina, Jérôme Nigou, Anne Lemassu, and Mamadou Daffé
5. Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria
Yunlong Li, Pooja Keshavan, Jamie H. Corro, Ravi K. Koripella, Rajendra K. Agrawal, and Anil K. Ojha
6. Macrophage Infection Models for Mycobacterium tuberculosis
Benjamin K. Johnson, Sean M. Thomas, Andrew J. Olive, and Robert B. Abramovitch
7. Novel Single-Cell and High Throughput Microscopy Techniques to Monitor Dictyostelium discoideum-Mycobacterium marinum Infection Dynamics
Manon Mottet, Cristina Bosmani, Nabil Hanna, Jahn Nitschke, Louise Lefrancois, and Thierry Soldati
8. Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy
Giulia Manina and Neeraj Dhar
9. Measuring Efflux and Permeability in Mycobacteria
Liliana Rodrigues, José A. AÃnsa, and Miguel Viveiros
10. Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria
Savannah E.R. Gibson, James Harrison, and Jonathan A.G. Cox
11. Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells
Sydney L. Solomon and Bryan D. Bryson
12. Electroporation of Mycobacteria
Tanya Parish
13. Gene Switching and Essentiality Testing
Amanda Claire Brown
14. Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis
Kenan C. Murphy
15. Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action
Eachan O. Johnson and Deborah T. Hung
16. CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria
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