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Molecular Toxicology Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1105
Medical ReferenceOther Animals
ISBN13: 9781627037389
Publisher: Humana
Published: Mar 13 2014
Pages: 646
Weight: 2.95
Height: 1.60 Width: 7.30 Depth: 10.10
Language: English
Part I. Toxicoproteomics
1. Array-Based Immunoassays with Rolling-Circle Amplification Detection
Katie Partyka, ShuangshuangWang, Ping Zhao, Brian Cao, and Brian Haab
2. Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis
Weimin Gao
Part II. Toxicant-Induced Inflammation
3. Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage
Yuanpu Peter Di
4. Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System
Yingze Zhang, Rahel Birru, and Y. Peter Di
Part III. Gene Promoter Methylation
5. Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques
Hang-Kai Hsu, Yu-I Weng, Pei-Yin Hsu, Tim H.-M. Huang, and Yi-Wen Huang
6. Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)
Kamaleshwar P. Singh
Part IV. Array Technologies
7. Strategies for Measurement of Biotransformation Enzyme Gene Expression
Marjorie Romkes and Shama C. Buch
8. Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening
Marjorie Romkes and Shama C. Buch
9. TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material
Kaori Shintani-Ishida, Bao-Li Zhu, and Hitoshi Maeda
Part V. Analysis of DNA Adducts
10. 32P-Postlabelling Analysis of DNA Adducts
David H. Phillips and Volker M. Arlt
11. Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot
Masako Ochiai, Takashi Sugimura, and Minako Nagao
12. DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry
Karen H. Dingley, Esther A. Ubick, John S. Vogel, Ted J. Ognibene, Michael A. Malfatti, Kristen Kulp, and Kurt W. Haack
13. Analysis of DNA Strand Cleavage at Abasic Sites
Walter A. Deutsch and Vijay Hegde
Part VI. Detection of Chromosomal and Genome-Wide Damage
14. Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes
Rupak Pathak and Pataje G. S. Prasanna
15. Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk
Xifeng Wu, Yun-Ling Zheng, and T. C. Hsu
16. Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair
Nina Joshi and Stephen G. Grant
Part VII. Detection and Characterization of Surrogate Gene Mutation
17. Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice
Vasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, and Robert H. Heflich
18. The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay
Nicole T. Myers and Stephen G. Grant
19. Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice
Seishi Kyoizumi, Yoichiro Kasunoki, and Tomonori Hayashi
20. Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes
Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich
21. Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems
Roy R. Swiger
22. The Human T-Cell Cloning Assay: Ide
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