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Mitochondrial Regulation: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2310
ISBN13: 9781071614358
Publisher: Humana
Published: Jun 7 2021
Pages: 318
Weight: 1.27
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
1. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression
André L. Simão, Pedro M. Borralho, Clifford J. Steer, Rui E. Castro, and CecÃlia M.P. Rodrigues
2. Determination of Oxidative Phosphorylation Complexes Activities
João S. Teodoro, Ivo F. Machado, Carlos M. Palmeira, and Anabela P. Rolo
3. BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function
Elena Silvestri, Assunta Lombardi, Federica Cioffi, and Fernando Goglia
4. Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs)
Bibiana Correia, Maria Inês Sousa, Ana F. Branco, and João Ramalho-Santos
5. Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes
Renata S. Tavares, Sara-Escada-Rebelo, Maria M. Soares, Andreia F. Silva, Teresa Almeida-Santos, Sandra Amaral, Ana Paula Sousa, and João Ramalho-Santos
6. Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections
Mariusz R. Wieckowski, Maciej Pronicki, and Agnieszka Karkucinska-Wieckowska
7. An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies
Magdalena Lebiedzinska-Arciszewska, Lech Wojtczak, and Mariusz R. Wieckowski
8. PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species
Tess C. Leuthner, Jessica H. Hartman, Ian Ryde, and Joel N. Meyer
9. Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases
Simone Patergnani, Massimo Bonora, Esmaa Bouhamida, Alberto Danese, Saverio Marchi, Giampaolo Morciano, Maurizio Previati, Gaia Pedriali, Alessandro Rimessi, Gabriele Anania, Carlotta Giorgi, and Paolo Pinton
10. Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria
Ignacio Vega-Naredo, Gabriela Oliveira, Teresa Cunha-Oliveira, Teresa Serafim, Vilma A. Sardao, and Paulo J. Oliveira
11. Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry
Emilio Molina-Molina, Harshitha Shanmugam, Domenica Di Palo, Ignazio Grattagliano, and Piero Portincasa
12. Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD)
Ignazio Grattagliano, Agostino Di Ciaula, Jacek Baj, Emilio Molina-Molina, Harshitha Shanmugam, Gabriella Garruti, David Q.-H. Wang, and Piero Portincasa
13. The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria
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