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Microfluidic Techniques: Reviews and Protocols

Microfluidic Techniques: Reviews and Protocols

Paperback

Series: Methods in Molecular Biology, Book 321

Medical ReferenceBiology

ISBN10: 1617376132
ISBN13: 9781617376139
Publisher: Springer Nature
Published: Dec 9 2010
Pages: 248
Weight: 0.85
Height: 0.59 Width: 6.00 Depth: 9.00
Language: English
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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