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Method Development and Validation for Voriconazole Estimation

Method Development and Validation for Voriconazole Estimation

Paperback

Medical Reference

ISBN10: 3330051337
ISBN13: 9783330051331
Publisher: LAP Lambert Academic Publishing
Published: Nov 27 2024
Pages: 80
Weight: 0.28
Height: 0.19 Width: 6.00 Depth: 9.00
Language: English
A rapid, sensitive, and specific RP-HPLC method with UV detection was developed and validated for the determination of voriconazole. Chromatography was performed on an Enable C18 G column (250 × 4.6 mm) in isocratic mode, using a mobile phase of methanol and acetonitrile (60:40, v/v). The flow rate was set at 1 mL/min, and the effluent was monitored at 223 nm. Chromatographic analysis revealed a main peak for voriconazole at a retention time of 2.841 minutes. Methanol was selected as the solvent for the analysis, with the λmax determined to be 223 nm. The method exhibited linearity over a concentration range of 5-30 g/mL. Validation parameters, including linearity, range, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ), were assessed and met the required standards.

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Srinivasa Rao, Yarguntla

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Medical Reference