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Liver Stem Cells: Methods and Protocols

Liver Stem Cells: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 826

Biology

ISBN10: 1617794678
ISBN13: 9781617794674
Publisher: Springer Nature
Published: Dec 14 2011
Pages: 226
Weight: 1.30
Height: 0.80 Width: 6.90 Depth: 10.20
Language: English

Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stem cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells Methods and Protocols, expert researchers focus on several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and application of liver stem cells for cell therapy. These topics shed light on stem cell technology which may lead to the development of effective clinical modalities for human liver diseases. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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