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Lipid Signaling Protocols

Lipid Signaling Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1376

BiologyChemistry

ISBN10: 1493931695
ISBN13: 9781493931699
Publisher: Springer Nature
Published: Nov 10 2015
Pages: 257
Weight: 1.52
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English

In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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