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Legionella: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1921
ISBN13: 9781493990474
Publisher: Humana
Published: Jan 29 2019
Pages: 476
Weight: 2.39
Height: 1.06 Width: 7.00 Depth: 10.00
Language: English
Part I: Ecology, Physiology and Genetics of Legionella
1. Analysis of Iron Requirements and Siderophore Production
Denise M. Burnside, and Nicholas P Cianciotto
2. The Pathometabolism of Legionella Studied by Isotopologue Profiling
Klaus Heuner, Mareike Kunze, Fan Chen, and Wolfgang Eisenreich
3. Quantification of Viable but Non-culturable Cells of Legionella pneumophila
Rosalie S. Braun, Nilmini Mendis, Laam Li, and Sebastien P. Faucher
4. Selection of Legionella Virulence-related Traits by Environmental Protozoa
Francisco Amaro and Howard Shuman
5. Migration of Acanthamoeba castellanii through Legionella Biofilms
Ramon Hochstrasser and Hubert Hilbi
6. Scar-free Genome Editing in Legionella pneumophila
Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, and Christophe Gilbert
7. Querying Legionella Genomes using Transposition-sequencing
Léo Hardy and Xavier Charpentier
8. Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq
Stephanie R. Shames
9. Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae
Elizabeth Korevaar, Chen Ai Khoo, and Hayley J. Newton
Part II: Cellular Microbiology of Legionella
10. Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium
Amanda Welin, Stephen Weber, and Hubert Hilbi
11. Sorting of Phagocytic Cells Infected with Legionella pneumophila
Monica Rolando and Carmen Buchrieser
12. Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment
Nicolas Personnic, Bianca Striednig, and Hubert Hilbi
13. Mitochondrial Dynamics and Activity in Legionella-Infected Cells
Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, and Pedro Escoll
14. Perturbation of Legionella Cell Infection by RNA Interference
Bernhard Steiner, A. Leoni Swart, and Hubert Hilbi
Part III: Biochemical Assays to Study Legionella Effectors and Enzymes
15. Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila
Tomoko Kubori and Hiroki Nagai
16. In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography
Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, and Grant J. Jensen
17. Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins
Jiazhang Qiu and Zhao-Qing Luo
18. Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila
Nadezhda Levanova, Irina Tabakova, Thomas Jank, and Yury Belyi
19. Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification
Ernest C. So, Aurélie Mousnier, Gad Frankel, and Gunnar N. Schroeder
20. Inflammasome Activation in Legionella-infected Macrophages
Danielle P. A. Mascarenhas and Dario S. Zamboni
Part IV. Immunity and Host Response Against Legionella
21. Infection of Human Lung Tissue ExAlso in
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