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In Vitro Mutagenesis Protocols

In Vitro Mutagenesis Protocols

Hardcover

Series: Methods in Molecular Biology, Book 634

Medical ReferenceChemistryGenetics

ISBN10: 1607616513
ISBN13: 9781607616511
Publisher: Springer Nature
Published: Jul 12 2010
Pages: 442
Weight: 2.20
Height: 1.20 Width: 7.10 Depth: 10.10
Language: English

In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

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