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Immune Checkpoint Blockade: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1913
ISBN13: 9781493989782
Publisher: Humana Pr
Published: Jan 22 2019
Pages: 225
Weight: 1.40
Height: 0.56 Width: 7.00 Depth: 10.00
Language: English
Part I: Generating Hypothesis
1. Double Immunohistochemistry and Digital Image Analysis
Pablo Moreno-Ruiz, Lina Wik Leiss, Artur Mezheyeuski, and Monika Ehnman
2. Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells
Claudia Wickenhauser, Daniel Bethmann, Zipei Feng, Shawn M. Jensen, Carmen Ballesteros-Merino, Chiara Massa, Andre Steven, Marcus Bauer, Peter Kaatzsch, Nikolaos Pazaitis, Georgiana Toma, Carlo B. Bifulco, Bernard A. Fox, and Barbara Seliger
3. Systems Level Immune Monitoring by Mass Cytometry
Tadepally Lakshmikanth and Petter Brodin
4. Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry
Neo Shiyong, Aine O'Reilly, and Yago Pico de Coaña
5. High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry
Fabio Marino, Chloe Chong, Justine Michaux, and Michal Bassani-Sternberg
6. Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification
Uta Sandy Tretbar, Michael Friedrich, Maria-Filothei Lazaridou, and Barbara Seliger
Part II: Functional Assays
7. Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors
Stina Wickström and Tanja Lövgren
8. Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies
Nataly Cruz RodrÃguez, Johanna Lineros, Carol Stefany RodrÃguez, Lina MarÃa MartÃnez, and Josefa Antonia RodrÃguez
9. Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes
Jeroen Melief, Stina Wickström, Rolf Kiessling, and Yago Pico de Coaña
10. Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry
Alexander Martens, Graham Pawelec, and Christopher Shipp
11. Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro
Dhifaf Sarhan and Jeffrey S. Miller
12. Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay
Irene van der Haar Avila, Patricia Marmol, Rolf Kiessling, and Yago Pico de Coaña
13. Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry
Irene van der Haar Avila, Patricia Marmol, Jeannette Cany, Rolf Kiessling, and Yago Pico de Coaña
Part III: Animal Models
14. Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models
Krist
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