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Produzione e purificazione della laccasi dai funghi della carie bianca
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ISBN10: 6209428614
ISBN13: 9786209428616
Publisher: Edizioni Sapienza
Published: Feb 13 2026
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786209428616
Publisher: Edizioni Sapienza
Published: Feb 13 2026
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Italian
I funghi della carie bianca sono unici per la loro forte capacità di degradare in modo estensivo le sostanze xenobiotiche e gli inquinanti recalcitranti e sono stati ampiamente applicati nel biorisanamento e nella biotecnologia ambientale. Questi funghi secernono l'enzima extracellulare laccasi, che svolge un ruolo importante nella degradazione delle sostanze xenobiotiche. Nel presente studio è stata testata la capacità del basidiomicete della carie bianca Trametes versicolor NCIM 1086 di produrre laccasi su residui agricoli naturali. La fibra di palma è stata selezionata come substrato per la sua elevata attività enzimatica di picco; l'attività massima della laccasi è stata ottenuta in studi preliminari incubando beute contenenti fibra di palma con un contenuto iniziale di umidità dell'80% (pH 4,0) come substrato integrato con mannitolo (1,0% p/v), peptone (1% p/v), CuSO4 (1mM) e 4 ml di sospensione conidiale di T.versicolor per 7 giorni a 300C. Il volume dell'inoculo, il contenuto iniziale di umidità , la concentrazione del supplemento di carbonio e la concentrazione di CuSO4 sono stati selezionati per l'ottimizzazione mediante Central Composite Design (CCD), che ha mostrato un effetto significativo sulla produzione di laccasi negli studi preliminari. Sono stati condotti studi di decolorazione su coloranti tessili effluenti mediante laccasi parzialmente purificata.
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