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Inclusion Bodies: Methods and Protocols

Inclusion Bodies: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 2617

Technology & EngineeringBiologyChemistry

ISBN10: 1071629298
ISBN13: 9781071629291
Publisher: Humana
Published: Jan 20 2023
Pages: 272
Weight: 1.57
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Biology