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In Vitro Neurotoxicology: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 758
Medical ReferenceWomen's Health
ISBN13: 9781493958580
Publisher: Humana
Published: Aug 23 2016
Pages: 415
Weight: 1.74
Height: 0.93 Width: 7.00 Depth: 10.00
Language: English
1. In Vitro Neurotoxicology: An Introduction
Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti
Part I: Model Systems
2. Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies
Gennaro Giordano and Lucio G. Costa
3. Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures
Evelyn Tiffany-Castiglioni, Yongchang Qian, Rola Barhoumi, and Ying Zheng
4. Microglia Cell Culture: A Primer for the Novice
Anke Witting and Thomas Möller
5. Neural Stem Cells for Developmental Neurotoxicity Studies
Roshan Tofighi, Michaela Moors, Raj Bose, Wan Norhamidah Wan Ibrahim, and Sandra Ceccatelli
6. Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures
Paul Honegger, Antoinette Defaux, Florianne Monnet-Tschudi, and Marie-Gabrielle Zurich
7. Neurospheres as a Model for Developmental Neurotoxicity Testing
Ellen Fritsche, Kathrin Gassmann, and Timm Schreiber
8. Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures
Pamela J. Lein, Christopher D. Barnhart, and Isaac N. Pessah
9. Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity
Mariaserena Boraso and Barbara Viviani
10. In Vitro Models to Study the Blood Brain Barrier
Hilary Vernon, Katherine Clark, and Joseph P. Bressler
Part II: Cell Death
11. Measurements of Cell Death in Neuronal and Glial Cells
Gennaro Giordano, Sungwoo Hong, Elaine M. Faustman, and Lucio G. Costa
12. Measurements of Neuronal Apoptosis
Gennaro Giordano and Lucio G. Costa
13. Measurement of Isoprostanes as Markers of Oxidative Stress
Dejan Milatovic, Thomas J. Montine, and Michael Aschner
14. Assessment of Glutathione Homeostasis
Gennaro Giordano, Collin C. White, and Lucio G. Costa
15. Assessing Neuronal Bioenergetic Status
Stephanie L.H. Zeiger, Jeannette N. Stankowski, and BethAnn McLaughlin
Part III: Signaling in Neurotoxicity
16. Fluorescent Assessment of Intracellular Calcium in Individual Cells
David P. Cox, Collin C. White, and Terrance J. Kavanagh
17. Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures
Victor Briz and Cristina Suñol
18. Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues
Ronald B. Tjalkens, David L. Carbone, and Guoyao Wu
19. JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons
Won-Seok Choi, Heather M Klintworth, and Zhengui Xia
20. Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration
Faneng Sun, Anumantha G. Kanthasamy, and Arthi Kanthasamy
21. Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo
Prasada Rao S. Kodavanti
22. Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons
Prasada Rao S. Kodavanti
Part IV: Other End-Points
23.
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