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Immune Homeostasis: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 979
ISBN13: 9781627032896
Publisher: Springer Nature
Published: Feb 9 2013
Pages: 215
Weight: 1.25
Height: 0.70 Width: 7.20 Depth: 10.00
Language: English
1. Quantitating Lymphocyte Programmed Cell Death (PCD) In Vitro Using Simple Kill Assays
Lixin Zheng
2. Fluoresence-Activated Cell Sorting (FACS)-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death (RICD) in Activated, Primary Human T Cells
Gil Katz and Andrew L. Snow
3. Evaluation of IL-2-Withdrawal Induced Apoptosis in Human T Lymphocytes
Joao Bosco Oliveira
4. Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique
Bernice Lo and Madhu Ramaswamy
5. Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation
Nelia Cordeiro and Nicolas Bidère
6. Analyses of Programmed Cell Death in Dendritic Cells
Min Chen, Lily Huang, and Jin Wang
7. Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity
Francis Ka-Ming Chan, Kenta Moriwaki, and MarÃa José De Rosa
8. Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers
Sema Kurtulus and David Hildeman
9. Homeostatic Proliferation of Mature T Cells
Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, and Charles D. Surh
10. Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers
Liset Westera, Yan Zhang, Kiki Tesselaar, José A.M. Borghans, and Derek C. Macallan
11. Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis
Menno C. van Zelm, Magdalena A. Berkowska, Mirjam van der Burg, and Jacques J.M. van Dongen
12. Molecular Measurement of T Cell Receptor Excision Circles
Heather E. Lynch and Gregory D. Sempowski
13. Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis
R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, and W. Nicholas Haining
14. Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders
Yu Zhang and Helen C. Su
15. Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)
Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich
16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells
Dat Q. Tran
4. Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique
Bernice Lo and Madhu Ramaswamy
5. Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation
Nelia Cordeiro and Nicolas Bidère
6. Analyses of Programmed Cell Death in Dendritic Cells
Min Chen, Lily Huang, and Jin Wang
7. Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity
Francis Ka-Ming Chan, Kenta Moriwaki, and MarÃa José De Rosa
8. Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers
Sema Kurtulus and David Hildeman
9. Homeostatic Proliferation of Mature T Cells
Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, and Charles D. Surh
10. Quantitating Lymphocyte Homeostasis In Vivo in Humans U
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