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Imaging Flow Cytometry: Methods and Protocols

Imaging Flow Cytometry: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 1389

Medical Reference

ISBN10: 1493949012
ISBN13: 9781493949014
Publisher: Humana
Published: Aug 23 2016
Pages: 295
Weight: 1.20
Height: 0.65 Width: 7.00 Depth: 10.00
Language: English

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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