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Human T-Lymphotropic Viruses: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1582
ISBN13: 9781493968701
Publisher: Humana
Published: Mar 30 2017
Pages: 220
Weight: 1.38
Height: 0.56 Width: 7.00 Depth: 10.00
Language: English
Part I: Epidemiology and Transmission
1. Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection
Olivier Cassar and Antoine Gessain
2. Molecular Epidemiology Database for Sequence Management and Data Mining
Thessika Hiala Almeida Araújo, Filipe Ferreira de Almeida Rego, and Luiz Carlos Junior Alcantara
3. Reporter Systems to Study HTLV-1 Transmission
4. Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells
Sandrine Alais, Hélène Dutartre, and Renaud Mahieux
5. Isolation of Exosomes from HTLV-Infected Cells
Robert A. Barclay, Michelle L. Pleet, Yao Akpamagbo, Kinza Noor, Allison Mathiesen, and Fatah Kashanchi
Part II: Promoter Activity of HTLVs' Proteins
6. A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins
Elisa Bergamo, Erika Diani, Umberto Bertazzoni, and Maria Grazia Romanelli
7. Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2
Fabio Bignami, Riccardo Alessio Sozzi, Umberto Bertazzoni, and Elisabetta Pilotti
8. EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway
Colin Murphy and Noreen Sheehy
Part III: Genotyping and Gene Expression
9. Methods for Identifying and Examining HTLV-1 HBZ Post-Translational Modifications
Jacob Al-Saleem, Mamuka Kvaratskhelia, and Patrick L. Green
10. High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites
11. STR Profiling of HTLV-1-Infected Cell Lines
Vittoria Raimondi, Sonia Minuzzo, Vincenzo Ciminale, and Donna M. D'Agostino
12. Expression of HTLV-1 Genes in T-cells Using RNA Electroporation
Mariangela Manicone, Francesca Rende, Ilaria Cavallari, Andrea K. Thoma-Kress, and Vincenzo Ciminale
Part IV: Cellular Dynamics
13. Quantification of Cell Turnover in the Bovine Leukemia Virus Model
Alix de Brogniez, Pierre-Yves Barez, Alexandre Carpentier, Geronimo Gutierrez, Michal Reichert, Karina Trono, and Luc Willems
14. Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection
Federica Bozzano, Francesco Marras, and Andrea De Maria
Part V: Therapy
15. Overview of Targeted Therapies for Adult T-Cell Leukemia/Lymphoma
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