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Human Cytomegaloviruses: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2244
ISBN13: 9781071611104
Publisher: Humana
Published: Feb 9 2021
Pages: 468
Weight: 2.20
Height: 1.10 Width: 7.70 Depth: 10.20
Language: English
1. Overview of Human Cytomegalovirus Pathogenesis
Heather L. Fulkerson, Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko
2. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies
Giada Frascaroli and Christian Sinzger
3. Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection
Elizabeth A. Fortunato
4. The Use of Primary Human Cells to Analyze Human Cytomegalovirus Biology
Emma Poole, Ian Groves, Sarah Jackson, Mark Wills, and John Sinclair
5. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation
Megan Peppenelli, Jason Buehler, and Felicia Goodrum
6. Collection and Isolation of CD14+ Primary Human Monocytes via Dual Density Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis
Bailey S. Mosher, Heather Fulkerson, and Andrew D. Yurochko
7. Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors
Anne-Charlotte Stilp, Patrick König, Myriam Scherer, and Thomas Stamminger
8. Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis
M. Zeeshan Chaudhry, Martin Messerle, and Luka Cicin-Sain
9. Methods for Studying the Function of Cytomegalovirus GPCRs
Christine M. O'Connor and William E. Miller
10. Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions
Jamil Mahmud and Gary C. Chan
11. Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT
Iris K.A. Jones and Daniel N. Streblow
12. Use of a Phosphoproteomic Screen to Profile Early Changes during HCMV Infection of Human Monocytes
Liudmila S. Chesnokova and Andrew D. Yurochko
13. A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells
Xiaofei E and Timothy F. Kowalik
14. Quantitative Electron Microscopy to Study HCMV Morphogenesis
Clarissa Read, Paul Walther, and Jens von Einem
15. Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA)
Vivian P. Young, Margarette C. Mariano, Lionel Faure, and Juliet V. Spencer
16. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs
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