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Human Cytomegaloviruses: Methods and Protocols

Human Cytomegaloviruses: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 1119

Medical Reference

ISBN10: 162703787X
ISBN13: 9781627037877
Publisher: Springer Nature
Published: Mar 18 2014
Pages: 368
Weight: 1.85
Height: 1.00 Width: 7.10 Depth: 10.10
Language: English

With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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