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Helicobacter Species: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 921
ISBN13: 9781627030045
Publisher: Springer Nature
Published: Sep 27 2012
Pages: 258
Weight: 1.50
Height: 0.90 Width: 7.10 Depth: 10.10
Language: English
1. Introduction
JeanMarie Houghton
2. Helicobacter pylori: An Overview
Jennifer M. Noto and Richard M. Peek, Jr.
3. Perspectives on Methodology for in vitro Culture of Helicobacter pylori
Timothy L. Cover
4. Successful Culture Techniques for Helicobacter Species: General Culture Techniques for Helicobacter pylori
Jeannette M. Whitmire and D. Scott Merrell
5. Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents
Jeannette M. Whitmire and D. Scott Merrell
6. Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis
Jeannette M. Whitmire and D. Scott Merrell
7. The Helicobacter pylori cag Pathogenicity Island
Jennifer M. Noto and Richard M. Peek, Jr.
8. Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori
Jennifer M. Noto and Richard M. Peek, Jr.
9. A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori
Marina Leite and Ceu Figueiredo 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization Deepa Raju, David Rizzuti and Nicola L. Jones 11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence Deepa Raju, David Rizzuti, Nicola L. Jones 12. Rodent Models of Helicobacter Infection, Inflammation and Disease Songhua Zhang and Steven F. Moss 13. Bacterial Culture and Inoculation of Mice (Simple Infection) Brian M. Gray and Kathryn A. Eaton 14. Adoptive Transfer of Splenocytes to Immunocompromised Mice Brian M. Gray and Kathryn A. Eaton 15. Isolation of Gastric Lamina Propria Leukocytes (LPL) Brian M. Gray and Kathryn A. Eaton 16. Delayed-type Hypersensitivity Determination Brian M. Gray and Kathryn A. Eaton 17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression Brian M. Gray and Kathryn A. Eaton 18. Animal Models of Helicobacter Induced Disease - Methods to Successfully Infect the Mouse Nancy S. Taylor and James G. Fox 19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice Mark T Whary, Zhongming Ge and James G Fox 20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes Richard L. Ferrero, John E. Wilson, and Phil Sutton 21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections Arlin B. Rogers 22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models Arlin B. Rogers 23. Innate Immune Responses to Helicobacter pylori Infection- An Overview Milan K. Patel, Melanie I. Trombly and Evelyn A. Kurt-Jones 24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones 25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection 26. In vivo Measurement of Helicobacter pylori Infection Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand
Deepa Raju, David Rizzuti and Nicola L. Jones
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