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Hydrocarbon Degradation Potential of Halotolerant Bacteria: Catechol Metabolizing Enzymes

Hydrocarbon Degradation Potential of Halotolerant Bacteria: Catechol Metabolizing Enzymes

Paperback

Medical ReferenceBiology

ISBN10: 3656600120
ISBN13: 9783656600121
Publisher: Bod Third Party Titles
Published: Feb 28 2014
Pages: 64
Weight: 0.21
Height: 0.15 Width: 5.83 Depth: 8.27
Language: English
Scientific Study from the year 2013 in the subject Biology - Micro- and Molecular Biology, grade: A, Nirma University (Institute of Science), language: English, abstract: Hydrocarbon contamination of marine ecosystems has been a major environmental concern. Hydrocarbon metabolizing capacity of four halotolerant bacteria (Bacillus atrophaeus, Halomonas shengliensis, Halomonas koreensis, and Virgibacillus salarius) isolated from saline soil of Khambhat, India was investigated. Presence of catechol metabolizing enzymes (catechol 2,3 dioxygenase, chlorocatechol 1,2 dioxygenase, and protocatechuate 3,4 dioxygenase) was checked in V. salarius, as only this among all the test organisms could grow on the hydrocarbon substrates used, and compared with Pseudomonas oleovorans. Effect of salinity of the growth medium on activity of catechol metabolizing enzymes was also studied. Catechol 2,3 dioxygenase activity in both the organisms was more susceptible to increase in salinity of the growth medium than chlorocatechol 1,2-dioxygenase activity. To the best of our awareness, this is the first report of catechol metabolism in V. salarius. V. salarius was found to be capable of weak biofilm formation. As V. salarius is capable of growing at high salt concentration, alkaline pH, hydrocarbon degradation, and also of growth in presence of various metal ions, it can be an attractive candidate for bioremediation of marine oil spills. Organisms like V. salarius can also serve as a model for multiple stress tolerance in prokaryotes.

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