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Germinal Centers: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1623
ISBN13: 9781493983834
Publisher: Humana Pr
Published: Aug 2 2018
Pages: 338
Weight: 1.35
Height: 0.73 Width: 7.00 Depth: 10.00
Language: English
1. Analysis of the Germinal Center Reaction in Tissue Sections
David Dominguez-Sola and Giorgio Cattoretti
2. Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR
Yang Zhang, Laura Garcia-Ibanez, Geoffrey Brown, and Kai-Michael Toellner
3. Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy
Carolin Ulbricht, Randall L. Lindquist, Laura Tech, and Anja E. Hauser
4. Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation
Johanne T. Jacobsen and Gabriel D. Victora
5. Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-photon Photoconversion
Imogen Moran and Tri Giang Phan
6. Intravital Microscopy of T-B Cell Interactions in Germinal Centers
Changming Shih and Hai Qi
7. Identifying Follicular Regulatory T cells by Confocal Microscopy
Vanderleyden I, Linterman MA
8. Cytokine Expression by T follicular Helper Cells
Christoph Jandl, Claudia Loetsch, and Cecile King
9. Follicular Dendritic Cell Isolation and Loading of Immune Complexes
Balthasar A. Heesters, Cornelis E. Van der Poel, and Michael C. Carroll
10. Isolation and Characterization of Mouse and Human Follicular Dendritic Cells
Mohey Eldin M. El Shikh, Riham El Sayed, and Costantino Pitzalis
11. In-vitro-induced Germinal Center B Cell Culture System
Kei Haniuda, Takuya Nojima and Daisuke Kitamura
12. CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells
Van Trung Chu, Robin Graf and Klaus Rajewsky
13. Germinal Center Formation with Retrovirally-transduced B Cells for Determining the Role of Specific Molecules In Vivo
Rinako Nakagawa
14. Characterization of the B Cell Transcriptome by RNA-Binding Proteins using iCLIP
Manuel D. DÃaz-Muñoz, Elisa Monzón-Casanova, and Martin Turner
15. Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity
Davide Angeletti, Gregory M. Frank, and Jonathan W. Yewdell
16. Somatic Hypermutation and Affinity Maturation Analysis Using the 4-hydroxy-3-nitrophenyl-acetyl (NP) System
Nicole Heise and Ulf Klein
17. Targeting Gene Function in Germinal Center B Cells: A Practical Approach
Valentina Petrocelli and Stefano Casola
18. Development of Mouse Model Systems of Germinal Centre Lymphomas
Eleni Kabrani and Sandrine Sander
19. The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas
Simon Le Gallou, Takuya Nojima, Daisuke Kitamura, Jean-Claude Weill, and Claude-Agnès Reynaud
20. Determining the Origin of Human Germinal Center B Cell-derived Malignancies
Marc Seifert and Ralf Küppers
21. Tracking B-cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes
Nicola J. Weston-Bell, Graeme Cowan, and Surinder S. Sahota
22. How to Simulate a Germinal Centre
Philippe A. Robert, Ananya Rastogi, Sebastian Binder, and Michael Meyer-Hermann
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