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Genotoxicity Assessment: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1044
Medical ReferenceOther Animals
ISBN13: 9781627035286
Publisher: Humana
Published: Jul 30 2013
Pages: 463
Weight: 2.24
Height: 1.30 Width: 7.10 Depth: 10.00
Language: English
Part I: Gene Mutation Assays
1. Bacterial Mutation Assays
Errol Zeiger
2. In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay
Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi
3. Erythrocyte--Based Pig--a Gene Mutation Assay
Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger
4. Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat Lymphocytes
Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich
5. In vivo cII, gpt, and Spi- gene Mutation Assays in Transgenic Mice and Rats
Mugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, and Robert H. Heflich
Part II; Assays for Chromosomal Abnormalities
6. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests
Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, and Fabrizio Palitti
7. Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse
Francesca Pacchierotti and Valentina Stocchi
8. Chromosomal Aberration Test in Human Lymphocytes
Christian Johannes and Guenter Obe
9. In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood
Sawako Kasamoto, Shoji Masumori, and Makoto Hayashi
10. Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells
Claudia Bolognesi and Michael Fenech
11. Flow Cytometric Determination of Micronucleus Frequency
Azeddine Elhajouji and Magdalena Lukamowicz-Rajska
12. Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus Test
Ilse Decordier and Micheline Kirsch-Volders
13. Comparative Genomic Hybridization (CGH) Genotoxicology
Baumgartner A
14. The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity
Bella B. Manshian, Neenu Singh, and Shareen H. Doak
Part III: Tests for Primary DNA Damage
15. g--H2AX Detection in Somatic and Germ Cells of Mice
Eugenia Cordelli and Lorena Paris
16. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers
Julian Laubenthal, Michal R. Gdula, Alok Dhawan, and Diana Anderson
17. The Comet Assay: Assessment of in vitro and in vivo DNA Damage
Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan
18. The Comet Assay in Human Biomonitoring
Diana Anderson, Alok Dhawan, and Julian Laubenthal
19. The Comet assay in Marine Animals
G. Frenzilli and B.P. Lyons
20. Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo
Fabrice Nesslany
21. 32P--Postlabeling Analysis of DNA Adducts
Heinz H. Schmeiser, Marie Stiborova, and Volker M. Arlt
Part IV: Assays in Plants and Aleternate Animal Models
22. Micronucleus Assay with Tetrad Cells of Tradescantia
Miroslav MisÃk, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, and Siegfried Knasmueller
23. The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in Drosophila
Ricard Marcos and Erico R. Carmona
Part V: Guidelines
24. Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH)
Gireesh Kamath H. and K.S. Rao
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