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Gap Junction Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1437
ISBN13: 9781493936625
Publisher: Springer Nature
Published: May 21 2016
Pages: 235
Weight: 1.44
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
1. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction
Michaël Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken
2. DNA Methylation Analysis of Human Tissue-Specific Connexin Genes
Xiaoming Liu and Canxia Xu
3. Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis
Joost Willebrords, Michaël Maes, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken
4. Immunohisto- and Cytochemistry Analysis of Connexins
Bruno Cogliati, Michaël Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, and Mathieu Vinken
5. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells
Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson
6. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor
Mark Kibschull, Stephen J. Lye, and Oksana Shynlova
7. Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells
Alexander W. Lohman, Adam C. Straub, and Scott R. Johnstone
8. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis
Stephanie Fowler, Mark Akins, and Steffany A.L. Bennett
9. Scrape Loading/Dye Transfer Assay
Pavel Babica, Iva Sovadinová, and Brad L. Upham
10. Microinjection Technique for Assessment of Gap Junction Function
Michael D. Fridman, Jun Liu, Yu Sun, and Robert M. Hamilton
11. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling
Elke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert
12. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro
Maria Kuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley
13. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging
Song Yang and Wen-Hong Li
14. A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells
Joseph P. Stains and Roberto Civitelli
15. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels
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